Luciferin 4-monooxygenase (UniProt: P08659; also known as EC: 1.13.12.7, Luciferase) is a peroxisomal enzyme that belongs to the ATP-dependent AMP-binding enzyme family. It catalyzes the production of oxyluciferin from luciferin in an ATP and magnesium dependent reaction and releases a photon of light when oxyluciferin returns to the ground state. This reaction proceeds in two steps where the first step involves adenylation of luciferin and in the second step luciferase acts as an oxygenase on adenyl-luciferin to produce oxyluciferin and carbon dioxide, the decay of oxyluciferin producing a photon of light of 562 nm. Luciferase from the firefly is commonly used in reporter assay where the total light emission is proportional to the luciferase activity of the sample.，官网链接：https://www.sigmaaldrich.cn/product/mm/ab3256i 默克 科研、开发、生产。 作为生命科学行业的全球领先供应商，我们致力于为科研、生物技术开发和生产，以及制药药物疗法开发和生产提供各类解决方案和服务。

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