Gram staining has been the gold standard technique for bacterial classification based on the different physical properties of the bacterial cell‐wall structure. However, Gram staining requires several steps, that is, fixation of the bacterial cell, two steps of staining with two different dyes, followed by decolorization, and washing. Notably, the decolorization step often generates false Gram determination, and the washing step can cause significant cell loss. An alternative to the Gram staining method is a fluorescence‐based dye approach for flow cytometry or fluorescence microscopy.BioTracker BacGO Gram-positive Bacteria Dye is an orange colored dye that can be used to identify Gram-positive bacteria by flow cytometry and fluorescence imaging. BacGO dye has been tested using real samples of colony‐forming bacteria, activated bacterial sludge, and a bacterial eye infection model. To fully demonstrate the universal selectivity of BacGO dye, it was tested on 16 bacterial strains (9 Gram‐positive and 7 Gram-negative) and compared the results with Gram staining. BacGO stained all of the known Gram‐positive bacteria and the result was validated with Gram staining. The universal selectivity of BacGO was also confirmed by flow cytometry using DAPI to stain the nuclei of all the bacteria.Spectral properties:Excitation: 558 nmEmission: 573 nmResearch Category:Live Cell Dye，官网链接：https://www.sigmaaldrich.cn/product/mm/sct071 默克 科研、开发、生产。 作为生命科学行业的全球领先供应商，我们致力于为科研、生物技术开发和生产，以及制药药物疗法开发和生产提供各类解决方案和服务。

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