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SCT262 is a novel fluorescent probe for the detection of ferrous ion (Fe2+) in mitochondria where Fe-S clusters and heme proteins are synthesized, and enables live cell fluorescent imaging of intracellular Fe2+. The SCT262 probe has no chelating ability. Add 53 uL DMSO to the 50 ug probe in the tube to create 1mM stock solution. Dilute with HBSS to prepare 5uM working solution.Cells may be incubated for 30 mins in the working solution, after which the supernatant solution can be replaced with the desired treatment solution.SPECTRAL PROPERTIESλex: 505 nmλem: 535 nmSuitable for use with FITC/GFP laser or filter,官网链接:https://www.sigmaaldrich.cn/product/mm/sct262
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