Propidium iodide (PI) is often used for cell cycle analysis by flow cytometry. Compared to this analysis method, the BioTracker™ Far Red Live Cell Cycle Assay Solution does not require cell fixation or permeabilization, and is permeable in live cells. Use 5 uL of probe solution in 500 uL PBS for each sample. Vial contains enough for 50 tests.The far red probe uses the 633 laser, enabling multiplexing with shorter wavelength probes by freeing the 488 laser for probes that emit in the green range of the spectrum. IMPORTANT APPLICATION NOTES:*Wash cells thoroughly to remove trypsin. Residual trypsin can confound analysis of flow cytometry populations.*Adjust gates after cells are stained with probe SPECTRAL PROPERTIESExcitation: 633 - 647 nmEmission: 695 LP or 780/60 BP.A filter set for APC-Cy7 is suitable.，官网链接：https://www.sigmaaldrich.cn/product/mm/sct280 默克 科研、开发、生产。 作为生命科学行业的全球领先供应商，我们致力于为科研、生物技术开发和生产，以及制药药物疗法开发和生产提供各类解决方案和服务。

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