BioTracker™ EP-HMRG is nearly nonfluorescent at pH 7 or higher. It fluoresces by the reaction with proteases that specifically digest the EP (glutamic acid, proline) peptide sequence. A green fluorescent product is membrane permeable and localizes to the lysosome.To use the probe, warm the vial to room temperature and add 30 μL of DMSO or PBS to one vial to make 1 mM solution.Dilute the stock solution with a neutral buffer or culture medium to 1-2 uM and use the diluted solution immediately.SPECTRAL PROPERTIES:Excitation: 500 nmEmission: 524 nmFor fluorescence microscopy, blue excitation filter cubes for FITC or GFP are suitable.REFERENCEOnoyama H, Kamiya M, Kuriki Y, Komatsu T, Abe H, Tsuji Y, Yagi K, Yamagata Y, Aikou S, Nishida M, et al. 2016. Rapid and sensitive detection of early esophageal squamous cell carcinoma with fluorescence probe targeting dipeptidylpeptidase IV. Scientific Reports. 6(1):26399.，官网链接：https://www.sigmaaldrich.cn/product/mm/sct205 默克 科研、开发、生产。 作为生命科学行业的全球领先供应商，我们致力于为科研、生物技术开发和生产，以及制药药物疗法开发和生产提供各类解决方案和服务。

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