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The pET-32c(+) vector is designed for cloning and high-level expression of peptide sequences fused with the 109aa Trx•Tag thioredoxin protein (1). Cloning sites are available for producing fusion proteins also containing cleavable His•Tag and S•Tag sequences for detection and purification. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB122VM. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.,官网链接:https://www.sigmaaldrich.cn/product/mm/69017
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