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The pET-27b(+) vector carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal HSV•Tag and HIS TAG® sequences. This vector differs from pET-25b(+) only by its selectable marker (kanamycin vs. ampicillin resistance). Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB073).
The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.,官网链接:https://www.sigmaaldrich.cn/product/mm/69863m
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