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The pET-30 Xa/LIC vector is an Xa/LIC version of pET-30b(+). It is designed for cloning and high-level expression of target proteins fused with the HIS TAG® and S•Tag coding sequences that are cleavable with Factor Xa protease. The plasmid contains a strong T7lac promoter, an optimized RBS, the coding sequence for the Xa protease cleavage site (IleGluGlyArg↓), and a multiple cloning site that contains restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal HIS TAG coding sequence is compatible with purification, detection, and quantification...,官网链接:https://www.sigmaaldrich.cn/product/mm/70073m
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