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The pET Dsb Fusion Systems 39b and 40b are designed for cloning and high-level expression of peptide sequences fused with the 208-aa DsbA•Tag sequence [pET-39b(+)] or the 236-aa DsbC•Tag sequence [pET-40b(+)].
DsbA and DsbC are periplasmic enzymes that catalyze the formation and isomerization of disulfide bonds, respectively (Rietsch 1996, Sone 1997, Missiakas 1994, Zapun 195, Raina 1997). In contrast to the pET-32 Trx•Tag series, which is designed to increase solubility in the cytoplasm, the DsbA•Tag and DsbC•Tag vectors enable potential periplasmic localization and thus may enhance solubility and proper folding in this non-reducing environment (Collins-Racie 1995).
The pET-39b(+) vector is designed for expression of DsbA fusion proteins. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB230).
The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.,官网链接:https://www.sigmaaldrich.cn/product/mm/70090m
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