The Clonables 2X Ligation Premix is a single solution containing optimized concentrations of the highest quality T4 DNA ligase, buffer, stabilizer, and cofactors needed for efficient ligation of any type of compatible DNA ends. The premix is tested for ligation of compatible 2- to 4-base cohesive DNA ends as well as blunt ends and single-base overhangs found on some PCR products. Under most conditionsexcellent ligation is achieved in only 15 minutes; with T/A or U/A overhangs more colonies can be obtained by incubating up to two hours. Although maximal efficiencies are obtained using NovaBlue Singles Competent Cells, this reagent is compatible with transformation of any type of chemically competent cells provided by the user. The high performance, minimal pipetting requirement, and single-addition format make the premix suitable for high-throughput applications.，官网链接：https://www.sigmaaldrich.cn/product/mm/70573 默克 科研、开发、生产。 作为生命科学行业的全球领先供应商，我们致力于为科研、生物技术开发和生产，以及制药药物疗法开发和生产提供各类解决方案和服务。

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