pET Expression Systems and pET pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression. Components: pET Expression Systems Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include: 10 µg pET vector DNA (for each indicated plasmid) 0.2 ml BL21 Glycerol Stock ml BL21(DE3) Glycerol Stock 0.2 ml BL21(DE3)pLysS Glycerol Stock 0.2 ml Induction Control Glycerol Stock Components: pET Expression Systems plus Competent Cells pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description: 0.2 ml NovaBlue Competent Cells 0.2 ml BL21(DE3) Competent Cells 0.2 ml BL21(DE3)pLysS Competent Cells 2 × 0.2 ml SOC Medium 10 µl Test Plasmid These components are sufficient for up to 10 transformations in each host. Purification and Detection Reagents Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters. pET Expression System 3 The pET Expression System 3 contains 10 µg each of the four versions of pET-30 (pET-30a–c(+)). The pET-30a–c(+) vectors carry an N-terminal HIS TAG®/thrombin/S•Tag/enterokinase configuration plus an optional C-terminal HIS TAG sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB095VM.，官网链接：https://www.sigmaaldrich.cn/product/mm/70781 默克 科研、开发、生产。 作为生命科学行业的全球领先供应商，我们致力于为科研、生物技术开发和生产，以及制药药物疗法开发和生产提供各类解决方案和服务。

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