NovaTaq DNA polymerase is a recombinant form of Thermus aquaticus DNA polymerase. This enzyme is a non-proofreading DNA polymerase. NovaTaq DNA polymerase exhibits 5′-3′ DNA polymerase activity and lacks 3′- 5′ exonuclease activity. The preparation is >95% homogenous by sodium dodecyl sulfate-poly acrylamide gel electrophoresis (SDS-PAGE) and lacks detectable endonuclease activities. It is tested for several quality control assays. This enzyme produces PCR products with 3′-dA overhangs, suitable for cloning with Novagen®Perfectly Blunt®, AccepTor, and LIC Vector Kits. Each item also includes optimized 10X NovaTaq Buffer with 15 mM MgCl₂ for routine amplification conditions, plus separate vials of 10X NovaTaq Buffer without MgCl₂, and 25 mM MgCl₂ to enable convenient optimization of Mg²⁺ concentration. Source: Recombinant Thermus aquaticus DNA polymerase expressed in E. coli Concentration: 5.0 U/μl Purity: 95% homogeneous by SDS-PAGE Endonuclease: None detected Amplification effiency: Functional PCR Storage: –20°C，官网链接：https://www.sigmaaldrich.cn/product/mm/71003m 默克 科研、开发、生产。 作为生命科学行业的全球领先供应商，我们致力于为科研、生物技术开发和生产，以及制药药物疗法开发和生产提供各类解决方案和服务。

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