The pET-44 vectors are designed for cloning and high-level expression of polypeptide sequences fused with the 495 aa NusA (Nus•Tag) protein. The solubility of many target proteins in the E. coli cytoplasm is increased when they are expressed NusA fusions. The pET-44a-c(+) vectors, which are derived from the corresponding pET-43.1 series, encode an additional N-terminal His•Tag sequence for enhanced yields when purifying a target protein with His•Mag Agarose Beads from lysates generated with the BugBuster HT Reagent and PopCulture Reagent. N-terminal S•Tag and optional C-terminal HSV•Tag and His•Tag fusion sequences are also available for versatile detection and purification of target proteins.，官网链接：https://www.sigmaaldrich.cn/product/mm/71124 默克 科研、开发、生产。 作为生命科学行业的全球领先供应商，我们致力于为科研、生物技术开发和生产，以及制药药物疗法开发和生产提供各类解决方案和服务。

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