The pETcoco System combines the advantages of T7 promoter-driven proteinexpression with the ability to control plasmid copy number (Sektas 2003). Inthe presence of 0.2% glucose, pETcoco recombinant plasmids are maintained atone copy per cell. In this single-copy state, pETcoco clones are extremely stable becausebackground expression and consequent gene rearrangements or deletions are virtuallyabsent. For plasmid preparation, the copy number can be amplified to approximately 40copies per cell independently of target gene expression by addition of L-arabinose to theculture medium. Both target gene expression and plasmid plus insert amplificationare induced upon addition of IPTG. Both pETcoco-1 and pETcoco-2 vectors featurean N-terminal His•Tag and internal S•Tag sequences, both of which are cleavable by enterokinase, and an optional C-terminal HSV•Tag sequence. NovaF⁻ Competent Cells for cloning target genes and Tuner(DE3) Competent Cells for expression are supplied with the pETcoco-1 and pETcoco-2 Systems.，官网链接：https://www.sigmaaldrich.cn/product/mm/71131 默克 科研、开发、生产。 作为生命科学行业的全球领先供应商，我们致力于为科研、生物技术开发和生产，以及制药药物疗法开发和生产提供各类解决方案和服务。

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